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Product Description
Human PEDF (Pigment Epithelium Derived Factor) ELISA Kit | ELK2723 | ELK Biotechnology
Alternative Names: SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor, Clade F Member 1 (Alpha-2 Antiplasmin) ; Stromal Cell Derived Factor 3
Species: Human
Assay Type: Sandwich
Sensitivity: 1.25 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pigment Epithelium Derived Factor (PEDF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pigment Epithelium Derived Factor (PEDF) in the samples is then determined by comparing the OD of the samples to the standard curve.
