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Product Description
Human REG4 (Regenerating Islet Derived Protein 4) ELISA Kit | ELK2208 | ELK Biotechnology
Alternative Names: GISP; REG-IV; RELP; Regenerating Gene Type IV; Gastrointestinal Secretory Protein; REG-like protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Infection immunity;Endocrinology;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 4 (REG4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 4 (REG4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 4 (REG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 4 (REG4) in the samples is then determined by comparing the OD of the samples to the standard curve.
