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Product Description
Human SPD (Surfactant Associated Protein D) ELISA Kit | ELK1072 | ELK Biotechnology
Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D
Species: Human
Assay Type: Sandwich
Sensitivity: 0.233 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Pulmonology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein D (SPD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Surfactant Associated Protein D (SPD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein D (SPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein D (SPD) in the samples is then determined by comparing the OD of the samples to the standard curve.
