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Product Description
Mouse a2M (Alpha-2-Macroglobulin) ELISA Kit | ELK1324 | ELK Biotechnology
Alternative Names: α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 1.51 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Lenght: 2h
Research Area: Tumor immunity;Infection immunity;Hematology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-2-Macroglobulin (a2M) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin (a2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
