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Product Description
Mouse PP (Pancreatic Polypeptide) ELISA Kit | ELK3027 | ELK Biotechnology
Alternative Names: PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 8.39 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pancreatic Polypeptide (PP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pancreatic Polypeptide (PP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreatic Polypeptide (PP) in the samples is then determined by comparing the OD of the samples to the standard curve.
