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Product Description
Mouse AGGF1 (Angiogenic Factor With G Patch And FHA Domains 1) ELISA Kit | ELK8053 | ELK Biotechnology
Alternative Names: GPATC7; GPATCH7; VG5Q; Angiogenic factor VG5Q; G patch domain-containing protein 7; Vasculogenesis gene on 5q protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 34 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) in the samples is then determined by comparing the OD of the samples to the standard curve.
