Product Description
Mouse AVPR2 (Arginine Vasopressin Receptor 2) ELISA Kit | ELK7233 | ELK Biotechnology
Alternative Names: ADHR; DI1; DIR; DIR3; NDI; V2R; Nephrogenic Diabetes Insipidus; Vasopressin V2 receptor; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 2 (AVPR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 2 (AVPR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 2 (AVPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginine Vasopressin Receptor 2 (AVPR2) in the samples is then determined by comparing the OD of the samples to the standard curve.