Product Description
Mouse COL15 (Collagen Type XV) ELISA Kit | ELK6590 | ELK Biotechnology
Alternative Names: COL15A1; COL15-A1; Collagen Alpha-1 (XV) chain; Restin; Endostatin-XV; Related to endostatin;
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.106 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Developmental science;Nutrition metabolism;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XV (COL15) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type XV (COL15) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XV (COL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XV (COL15) in the samples is then determined by comparing the OD of the samples to the standard curve.