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Product Description
Mouse COL2a1 (Collagen Type II Alpha 1) ELISA Kit | ELK8627 | ELK Biotechnology
Alternative Names: COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1 (II) Chain; Primary Osteoarthritis, Spondyloepiphyseal Dysplasia, Congenital
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/L
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II Alpha 1 (COL2a1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type II Alpha 1 (COL2a1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II Alpha 1 (COL2a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II Alpha 1 (COL2a1) in the samples is then determined by comparing the OD of the samples to the standard curve.
