Product Description
Mouse CXCL15 (Chemokine C-X-C-Motif Ligand 15) ELISA Kit | ELK9605 | ELK Biotechnology
Alternative Names: C-X-C motif chemokine 15, Chemokine (C-X-C motif) ligand 15, Cxcl15, CXL15, Lungkine, Scyb15, Small inducible cytokine B15, Small inducible cytokine subfamily B member 15, Small-inducible cytokine B15, weche
Species: Mouse
Assay Type: Sandwich
Sensitivity: 46.88 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 15 (CXCL15) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 15 (CXCL15) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 15 (CXCL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Ligand 15 (CXCL15) in the samples is then determined by comparing the OD of the samples to the standard curve.