Euro
USD
British Pound
NULL
Product Description
Mouse CXCR3 (Chemokine C-X-C-Motif Receptor 3) ELISA Kit | ELK5018 | ELK Biotechnology
Alternative Names: CD183; CKR-L2; CMKAR3; GPR9; IP10r; IP10-R; Mig-R; MigR; G Protein-Coupled Receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 3 (CXCR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 3 (CXCR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Receptor 3 (CXCR3) in the samples is then determined by comparing the OD of the samples to the standard curve.
