Product Description
Mouse EMILIN2 (Elastin Microfibril Interface Located Protein 2) ELISA Kit | ELK6555 | ELK Biotechnology
Alternative Names: FOAP-10; Elastin Microfibril Interfacer 2
Species: Mouse
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 2 (EMILIN2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 2 (EMILIN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 2 (EMILIN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastin Microfibril Interface Located Protein 2 (EMILIN2) in the samples is then determined by comparing the OD of the samples to the standard curve.