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Product Description
Mouse F8 (Coagulation Factor VIII) ELISA Kit | ELK2752 | ELK Biotechnology
Alternative Names: FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 2.44 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: plasma
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Hematology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VIII (F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VIII (F8) in the samples is then determined by comparing the OD of the samples to the standard curve.
