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Product Description
Mouse GFAP (Glial Fibrillary Acidic Protein) ELISA Kit | ELK1628 | ELK Biotechnology
Alternative Names: Intermediate Filament Protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 24.9 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Fibrillary Acidic Protein (GFAP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glial Fibrillary Acidic Protein (GFAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Fibrillary Acidic Protein (GFAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Fibrillary Acidic Protein (GFAP) in the samples is then determined by comparing the OD of the samples to the standard curve.
