Product Description
Mouse GNaO1 (G Protein Alpha Activating Activity Polypeptide O) ELISA Kit | ELK7794 | ELK Biotechnology
Alternative Names: G-ALPHA-o; GNAO; Guanine nucleotide-binding protein G (o) subunit alpha
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha Activating Activity Polypeptide O (GNaO1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to G Protein Alpha Activating Activity Polypeptide O (GNaO1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha Activating Activity Polypeptide O (GNaO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Alpha Activating Activity Polypeptide O (GNaO1) in the samples is then determined by comparing the OD of the samples to the standard curve.