Product Description
Mouse IL35 (Interleukin 35) ELISA Kit | ELK5768 | ELK Biotechnology
Alternative Names: IL-35; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit ; IL-12 subunit p35
Species: Mouse
Assay Type: Sandwich
Sensitivity: 3.3 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 35 (IL35) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 35 (IL35) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 35 (IL35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 35 (IL35) in the samples is then determined by comparing the OD of the samples to the standard curve.