Euro
USD
British Pound
NULL
Product Description
Mouse REPIN1 (Replication Initiator 1) ELISA Kit | ELK6478 | ELK Biotechnology
Alternative Names: AP4; RIP60; ZNF464; Zfp464; Replication Initiation Region Protein (60kD) ; Zinc Finger Protein 464; 60 kDa origin-specific DNA-binding protein; ATT-binding protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.135 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1 (REPIN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Replication Initiator 1 (REPIN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Initiator 1 (REPIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Initiator 1 (REPIN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
