Product Description
Mouse TICAM1 (Toll Like Receptor Adaptor Molecule 1) ELISA Kit | ELK7262 | ELK Biotechnology
Alternative Names: TRIF; PRVTIRB; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; Proline-rich, vinculin and TIR domain-containing protein B
Species: Mouse
Assay Type: Sandwich
Sensitivity: 21.7 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor Adaptor Molecule 1 (TICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor Adaptor Molecule 1 (TICAM1) in the samples is then determined by comparing the OD of the samples to the standard curve.