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Product Description
Mouse TSPAN30 (Tetraspanin 30) ELISA Kit | ELK3340 | ELK Biotechnology
Alternative Names: CD63; LAMP-3; ME491; MLA1; OMA81H; Melanoma 1 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Ocular melanoma-associated antigen
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.063 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Hematology;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30 (TSPAN30) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetraspanin 30 (TSPAN30) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 30 (TSPAN30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetraspanin 30 (TSPAN30) in the samples is then determined by comparing the OD of the samples to the standard curve.
