Product Description
Pig ADST (Androstenone) ELISA Kit | ELK8586 | ELK Biotechnology
Alternative Names: Androstenone
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 10.19nmol/ml
Standard: 2000nmol/ml
Detection Range: 31.25-2000 nmol/ml
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Biochemical research
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androstenone (ADST) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androstenone (ADST) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androstenone (ADST) in the samples is then determined by comparing the OD of the samples to the standard curve.