Product Description
Pig IP3 (Inositol Triphosphate) ELISA Kit | ELK8873 | ELK Biotechnology
Alternative Names: InsP3; Inositol 1, 4, 5-Trisphosphate; Triphosphoinositol
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 49.7 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Inositol Triphosphate (IP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inositol Triphosphate (IP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Triphosphate (IP3) in the samples is then determined by comparing the OD of the samples to the standard curve.