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Product Description
Rat 8-iso-PGF2α (8-isoprostane) ELISA Kit | ELK9175 | ELK Biotechnology
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 9.375 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.625-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-isoprostane (8-iso-PGF2α) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-isoprostane (8-iso-PGF2α) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-isoprostane (8-iso-PGF2α) in the samples is then determined by comparing the OD of the samples to the standard curve.
