Product Description
Rat a1BG (Alpha-1-B-Glycoprotein) ELISA Kit | ELK8874 | ELK Biotechnology
Alternative Names: Alpha 1 B Glycoprotein; Alpha 1 beta Glycoprotein
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.147 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-B-Glycoprotein (a1BG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-B-Glycoprotein (a1BG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-B-Glycoprotein (a1BG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-B-Glycoprotein (a1BG) in the samples is then determined by comparing the OD of the samples to the standard curve.