Product Description
Rat aCTx (Alpha-Crosslaps) ELISA Kit | ELK1542 | ELK Biotechnology
Alternative Names: Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 43.45 pg/mL
Standard: 9000 pg/mL
Detection Range: 140.63-9000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Crosslaps (aCTx) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Crosslaps (aCTx) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Crosslaps (aCTx) in the samples is then determined by comparing the OD of the samples to the standard curve.