Product Description
Rat AhR (Aryl Hydrocarbon Receptor) ELISA Kit | ELK6229 | ELK Biotechnology
Alternative Names: BHLHE76; Class E basic helix-loop-helix protein 76
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.102 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor (AhR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor (AhR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor (AhR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor (AhR) in the samples is then determined by comparing the OD of the samples to the standard curve.