Product Description
Rat ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2) ELISA Kit | ELK6511 | ELK Biotechnology
Alternative Names: ATP6IP2; CAPER; ELDF10; XMRE; Renin/prorenin receptor; Embryonic liver differentiation factor 10; Vacuolar ATP synthase membrane sector-associated protein M8-9
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.128 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the OD of the samples to the standard curve.