Product Description
Rat BACE2 (Beta Secretase 2) ELISA Kit | ELK9252 | ELK Biotechnology
Alternative Names: AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP; Beta-Site APP Cleaving Enzyme 2; Aspartic-like protease 56 kDa; Aspartyl protease 1; Down region aspartic protease; Memapsin-1
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Neuroscience
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta Secretase 2 (BACE2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta Secretase 2 (BACE2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta Secretase 2 (BACE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta Secretase 2 (BACE2) in the samples is then determined by comparing the OD of the samples to the standard curve.