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Product Description
Rat CRH (Corticotropin Releasing Hormone) ELISA Kit | ELK2606 | ELK Biotechnology
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 4.95 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticotropin Releasing Hormone (CRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone (CRH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone (CRH) in the samples is then determined by comparing the OD of the samples to the standard curve.
