Product Description
Rat DSTYK (Dual Serine/Threonine And Tyrosine Protein Kinase) ELISA Kit | ELK7203 | ELK Biotechnology
Alternative Names: RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase 5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting serine/threonine-protein kinase 5
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) in the samples is then determined by comparing the OD of the samples to the standard curve.