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Product Description
Rat FXR (Farnesoid X Receptor) ELISA Kit | ELK6978 | ELK Biotechnology
Alternative Names: NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1, Group H, Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesoid X Receptor (FXR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Farnesoid X Receptor (FXR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesoid X Receptor (FXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesoid X Receptor (FXR) in the samples is then determined by comparing the OD of the samples to the standard curve.
