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Product Description
Rat IL15Ra (Interleukin 15 Receptor Alpha) ELISA Kit | ELK8781 | ELK Biotechnology
Alternative Names: CD215; IL15-RA; Interleukin 15 Receptor Alpha
Species: Rat
Assay Type: Sandwich
Sensitivity: 12.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 15 Receptor Alpha (IL15Ra) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 15 Receptor Alpha (IL15Ra) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 15 Receptor Alpha (IL15Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 15 Receptor Alpha (IL15Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.
