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Product Description
Rat KIF5B (Kinesin Family, Member 5B) ELISA Kit | ELK7128 | ELK Biotechnology
Alternative Names: KNS1; UKHC; KINH; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; Kinesin-1 heavy chain
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5B (KIF5B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kinesin Family, Member 5B (KIF5B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5B (KIF5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 5B (KIF5B) in the samples is then determined by comparing the OD of the samples to the standard curve.
