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Product Description
Rat NOX1 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1) ELISA Kit | ELK2600 | ELK Biotechnology
Alternative Names: MOX1; GP91-2; NOH1; NADPH Oxidase 1; Mitogenic Oxidase (Pyridine Nucleotide-Dependent Superoxide-Generating; NADH/NADPH mitogenic oxidase subunit P65-MOX
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.64 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Neuro science;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in the samples is then determined by comparing the OD of the samples to the standard curve.
