Product Description
Rat p-hydroxyphenyllactic acid (HPLA) ELISA Kit | ELK8872 | ELK Biotechnology
Alternative Names: HPLA
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 6.65ng/mL
Standard: 1000 ng/mL
Detection Range: 15.6-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: metabolize
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HPLA (p-hydroxyphenyllactic acid ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HPLA (p-hydroxyphenyllactic acid ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HPLA (p-hydroxyphenyllactic acid ) in the samples is then determined by comparing the OD of the samples to the standard curve.