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Product Description
Rat P4Ha1 (Prolyl-4-Hydroxylase Alpha Polypeptide I) ELISA Kit | ELK6135 | ELK Biotechnology
Alternative Names: Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Alpha Polypeptide I
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) in the samples is then determined by comparing the OD of the samples to the standard curve.
