Product Description
Rat PAI2 (Plasminogen Activator Inhibitor 2) ELISA Kit | ELK2583 | ELK Biotechnology
Alternative Names: SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Plasma
Assay Lenght: 3.5h
Research Area: Hematology;Reproductive science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 2 (PAI2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 2 (PAI2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 2 (PAI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasminogen Activator Inhibitor 2 (PAI2) in the samples is then determined by comparing the OD of the samples to the standard curve.