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Product Description
Rat PAR2 (Protease Activated Receptor 2) ELISA Kit | ELK1638 | ELK Biotechnology
Alternative Names: F2RL1; F2-RL1; GPR11; PAR2; Coagulation Factor II (thrombin) receptor-Like 1; G-protein coupled receptor 11; Thrombin receptor-like 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 2 (PAR2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protease Activated Receptor 2 (PAR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 2 (PAR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease Activated Receptor 2 (PAR2) in the samples is then determined by comparing the OD of the samples to the standard curve.
