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Product Description
Rat PC (Protein Carbonyl) ELISA Kit | ELK9078 | ELK Biotechnology
Alternative Names: Protein Carbonyl
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.114 nmol/mL
Standard: 20 nmol/mL
Detection Range: 0.32-20 nmol/mL
Sample Type: Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Hepatology;Hormone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Carbonyl (PC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Carbonyl (PC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Carbonyl (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Carbonyl (PC) in the samples is then determined by comparing the OD of the samples to the standard curve.
