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Product Description
Rat sC5b-9 (Soluble Terminal Complement Complex C5b-9) ELISA Kit | ELK9633 | ELK Biotechnology
Alternative Names: MAC, Membrane Attack Complex, C5b9, TCC C5b-9
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immunology；Metabolism；Signal Transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Terminal Complement Complex C5b-9 (sC5b-9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Terminal Complement Complex C5b-9 (sC5b-9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Terminal Complement Complex C5b-9 (sC5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Terminal Complement Complex C5b-9 (sC5b-9) in the samples is then determined by comparing the OD of the samples to the standard curve.
