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Product Description
Rat STAT2 (Signal Transducer And Activator Of Transcription 2) ELISA Kit | ELK7686 | ELK Biotechnology
Alternative Names: ISGF-3; P113; STAT113
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 2 (STAT2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 2 (STAT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 2 (STAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 2 (STAT2) in the samples is then determined by comparing the OD of the samples to the standard curve.
