Product Description
ERGIC53 Monoclonal Antibody | PM-7213 | ProSci
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Mouse monoclonal ERGIC53 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERGIC53.
Research Area: Homeostasis
Tested Application: E, WB, ICC
Application: ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Specificiy: At least two isoforms of ERGIC53 are known to exist.
Positive Control 1: Cat. No. 1209 - HL60 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 56 kDa
Validation: N/A
Isoform: N/A
Purification: ERGIC53 Monoclonal Antibody is affinity chromatography purified via peptide column.
Clonality: Monoclonal
Clone: N/A
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ERGIC53 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ERGIC53 Monoclonal antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1, LMAN1, endoplasmic reticulum-golgi intermediate compartment protein 53, ERGIC-53, F5F8D, FMFD1, MCFD1, MR60, gp58
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ERGIC53 Monoclonal Antibody: ERGIC53, also known as lectin mannose-binding 1 (LMAN1) protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.