Product Description
Anti- ALKBH1 Antibody | FNab00311 | FineTest
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Host: Rabbit
Clonality: polyclonal
Clone ID: N/A
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Dioxygenase that acts as a DNA demethylase (PubMed:18603530) . Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:18603530) . Specifically demethylates DNA methylated on the 6th position of adenine (N (6) -methyladenosine) DNA. N (6) -methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity) . Also able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation, but with low activity (PubMed:18603530) . Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed:19959401) . DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed:19959401, PubMed:23577621) . DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity) .
Immunogen: alkB, alkylation repair homolog 1 (E. coli)
synonyms: ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH
Molecular weight (observed) : 44 kDa
Reactivity Species: Human, Mouse, Rat
Tested Application: ELISA, IHC, WB
Recomended Dillution : WB: 1:200-1:2000; IHC: 1:20-1:200
Research Area: Metabolism