Product Description
Anti- UBE2K Antibody | FNab09178 | FineTest
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Host: Rabbit
Clonality: polyclonal
Clone ID: N/A
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
Immunogen: ubiquitin-conjugating enzyme E2K
synonyms: DKFZp564C1216, DKFZp686J24237, E2-25K, HIP 2, HIP2, HYPG, LIG, UBE2K, Ubiquitin carrier protein, Ubiquitin protein ligase
Molecular weight (observed) : 25 kDa
Reactivity Species: Human, Mouse, Rat
Tested Application: ELISA, WB, IHC, IF
Recomended Dillution : WB: 1:500-1:2000; IHC: 1:20-1:200
Research Area: Neuroscience, Immunology, Metabolism, Epigenetics