Product Description
Anti- UBE2O Antibody | FNab09181 | FineTest
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Host: Rabbit
Clonality: polyclonal
Clone ID: N/A
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950) . Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138) . Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153) . Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950) . Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853) .
Immunogen: ubiquitin-conjugating enzyme E2O
synonyms: E2-230K, FLJ12878, KIAA1734, UBE2O, Ubiquitin carrier protein O, Ubiquitin protein ligase O
Molecular weight (observed) : 141 kDa
Reactivity Species: Human, Mouse
Tested Application: ELISA, WB, IF
Recomended Dillution : WB: 1:200-1:2000
Research Area: Epigenetics, Metabolism