Product Description
Anti- HIF1a Antibody | FNab09940 | FineTest
Form: liquid
Purification: protein A+G purified
Purity: ≥95% as determined by SDS-PAGE
Host: Mouse
Clonality: monoclonal
Clone ID: 5H12
Isotype: IgG1
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918) .
Immunogen: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
synonyms: BHLHE78, MOP1, PASD8
Molecular weight (observed) : 120 kDa
Reactivity Species: Human
Tested Application: ELISA, WB, IHC, IF
Recomended Dillution : WB: 1:1000-1:10000; IF: 1:50-1:200
Research Area: Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells