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Product Description
Anti- TOP1MT Antibody | FNab08866 | FineTest
Form: liquid
Purification: Immunogen affinity purified
Purity: ≥95% as determined by SDS-PAGE
Host: Rabbit
Clonality: polyclonal
Clone ID: N/A
Isotype: IgG
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) .
Immunogen: topoisomerase (DNA) I, mitochondrial
synonyms: N/A
Molecular weight (observed) : 70 kDa
Reactivity Species: Human
Tested Application: ELISA, WB, IHC, IP
Recomended Dillution : WB: 1:500-1:2000
Research Area: Metabolism
![TOP1MT Antibody [TOP1MT/488]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575188/811586/porsci_lo__79508.1648973713__26506.1649091852.png?c=2 "TOP1MT Antibody [TOP1MT/488]")
