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Product Description
SSA ELISA kit | 55R-ORG652 | Fitzgerald
Activity Code: ACTIVE
Product Type: Research Kits
Product Subtype: Kits
Research Area: DNA & RNA
Short Description: ELISA kit for the detection of SSA in the research laboratory
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: N/A
Residues: N/A
Tag/conjugate: N/A
Protein Type: N/A
Expression System: N/A
Grade & Purity: N/A
Methode of Purification: N/A
Source: N/A
Concentration: N/A
Form & Buffer: N/A
Storage: Store at 2-8 deg C
Shipping Information: *Blue Ice*
Applications: ELISA
Bioactivity: N/A
Usage Recommendations: Optimal conditions to be determined by end-user
Assay Information: AntiSSA 52 is an indirect solid phase enzyme immunometric assay (ELISA) . It is designed for the quantitative measurement of IgG class autoantibodies directed against the extractable nuclear antigens SSA 52 (Ro 52) . The microplate is coated with SSA 52 (Ro 52) . The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway") . The binding of present autoantibodies, as well as the formation of the sandwich complexes and enzymatic color reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended. The optical density for each calibrator may be graphically plotted against the concentration of IgG and unknowns extrapolated from the curve.
