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  • Rabbit Anti-FN/Fibronectin 1 Polyclonal

    • Rabbit Anti-FN/Fibronectin 1 Polyclonal | 498-BS-0666R-A594

    $1,190

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    SKU:
    498-BS-0666R-A594
    Availability:
    IN STOCK
    Size:
    100 µL
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    Product Description

    Rabbit Anti-FN/Fibronectin 1 Polyclonal | 498-BS-0666R-A594

    biological source

    rabbit

    Quality Level

    200

    conjugate

    unconjugated

    antibody form

    affinity isolated antibody

    antibody product type

    primary antibodies

    clone

    polyclonal

    form

    buffered aqueous solution

    species reactivity

    human

    technique(s)

    indirect ELISA: 1:10,000 using human fibronectin
    indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts
    microarray: suitable
    western blot: 1:1,000 using human plasma fibronectin

    UniProt accession no.

    P02751

    Antiserum is produced in rabbit using purified human fibronectin as the immunogen. Affinity isolated antibody is obtained from antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to human fibronectin. The antiserum is determined to be immunospecific for human fibronectin by immunofluorescent labeling of human fibroblast cell cultures, ELISA and immunoblotting.

    In immunoblotting, a specific band of fibronectin at 220 kDa is observed (another band at 94 kDa may be also be present) using human fibronectin. When used in immunoelectrophoresis, the antibody shows 1-2 arcs of precipitation versus normal human plasma. This product may be used for immunohistochemical localization of fibronectin in normal, inflamed and neoplastic tissues, for detection of fibronectin on cultured cells and structure and function studies of fibronectins in human and animal body fluids, tissues and cells.

    Affinity isolated antibody to human fibronectin can be used for immunofluorescent and immunoperoxidase staining of cultured cells, frozen sections and formalin fixed, paraffin-embedded tissues. Other fixatives, e.g. methacarn and ethanol, may also be used.

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