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Product Description
Human Cell Line II Blot | 1502 | ProSci
Tested Application: WB
Application: For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Storage Condition: Store at 4°C. The blot can be reused after stripping.
User Note: Note: Lysate lane order may vary; please consult accompanying shipment paperwork.
Background: The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are Daudi (B lymphoblast; Burkkit's lymphoma), Raji (B lymphoblast; Burkkit's lymphoma), K-562 (bone marrow; chronic myelogenous leukmia), U-937 (histiocytic lymphoma), THP-1 (monocyte; acute monocytic leukmia), HL-60 (promyeloblast; acute promyeloblastic leukmia), Jurkat (T lymphocyte; acute T cell leukmia), and MOLT-4 (T lymphoblast; acute lymphoblastic leukmia) .
Shipping: N/A
Species: Human
Sample Loaded: Lanes: 1, protein markers; 2, Daudi; 3, Raji; 4, K-562; 5, U-937; 6, THP-1; 7, HL-60; 8, Jurkat; and 9, MOLT-4.
Quantity: 15 µg
Gel Percentage: Gradient 4-20%
Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs) .
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