Product Description
CDK4 Antibody [AMM02527G] | Leading Biology
Product Category: Monoclonal Antibodies
Host: Mouse
Species Reactivity: H, M
Specificity: This CDK4 antibody is generated from a mouse immunized with a recombinant protein.
Cellular Localisation: Cytoplasm. Nucleus. Nucleus membrane. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G (1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G (1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
Molecular Weight: 33730
Clone: Monoclonal
Gene Name: CDK4
Gene ID: 1019
Function: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Summary: N/A
Form: N/A
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB, IHC-P
Dilution: IHC-P--1:100~500 IHC--1:300 WB--1:2000
Synonyms: Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4